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Schizosaccharomyces japonicus Yukawa et Maki (1931) and Schizosaccharomyces versatilis Wickerham et Duprat (1945) have been treated as varieties of S. japonicus or as conspecific, based on various approaches including mating trials and nDNA/nDNA optical reassociation studies. However, the type strains of S. japonicus and S. versatilis differ by five substitutions (99.15% identity) and one 1‐bp indel...
In poor nitrogen conditions, fission yeast cells mate, undergo meiosis and form spores that are resistant to deleterious environments. Natural isolates of Schizosaccharomyces pombe are homothallic. This allows them to naturally switch between the two h− and h+ mating types with a high frequency, thereby ensuring the presence of both mating partners in a population of cells. However, alteration of...
Schizosaccharomyces japonicus belongs to the single‐genus class Schizosaccharomycetes, otherwise known as “fission yeasts.” As part of a composite model system with its widely studied S. pombe sister species, S. japonicus has provided critical insights into the workings and the evolution of cell biological mechanisms. Furthermore, its divergent biology makes S. japonicus a valuable model organism...
The fission yeast species Schizosaccharomyces japonicus is currently divided into two varieties—S. japonicus var. japonicus and S. japonicus var. versatilis. Here we examine the var. versatilis isolate CBS5679. The CBS5679 genome shows 88% identity to the reference genome of S. japonicus var. japonicus at the coding sequence level, with phylogenetic analyses suggesting that it has split from the S. japonicus...
Microfluidic chip for cell volume measurement using the Fluorescence Exclusion method. The chamber was filled with a fluorescent dye and Schizosaccharomyces pombe cells. Bright halos around the chamber pillars appeared after a few days in a used chip due to precipitation of the dye. D. García‐Ruano, I. Hsu, B. Leray, B. Billard, G. Liti, D. Coudreuse. Yeast, 41(3), 87‐94.
N6‐methyladenosine (m6A) is a highly abundant and evolutionarily conserved messenger RNA (mRNA) modification. This modification is installed on RRACH motifs on mRNAs by a hetero‐multimeric holoenzyme known as m6A methyltransferase complex (MTC). The m6A mark is then recognised by a group of conserved proteins known as the YTH domain family proteins which guide the mRNA for subsequent downstream processes...
Eukaryotic genes must be condensed into chromatin while remaining accessible to the transcriptional machinery to support gene expression. Among the three eukaryotic RNA polymerases (RNAP), RNAPII is unique, partly because of the C‐terminal domain (CTD) of its largest subunit, Rpb1. Rpb1 CTD can be extensively modified during the transcription cycle, allowing for the co‐transcriptional recruitment...
Polyadenylation occurs at numerous sites within 3′‐untranslated regions (3′‐UTRs) but rarely within coding regions. How does Pol II travel through long coding regions without generating poly(A) sites, yet then permits promiscuous polyadenylation once it reaches the 3′‐UTR? The cleavage/polyadenylation (CpA) machinery preferentially associates with 3′‐UTRs, but it is unknown how its recruitment is...
Genomes from yeast to humans are subject to pervasive transcription. A single round of pervasive transcription is sufficient to alter local chromatin conformation, nucleosome dynamics and gene expression, but is hard to distinguish from background signals. Size fractionated native elongating transcript sequencing (sfNET‐Seq) was developed to precisely map nascent transcripts independent of expression...
Transcription enables the production of RNA from a DNA template. Due to the highly dynamic nature of transcription, live‐cell imaging methods play a crucial role in measuring the kinetics of this process. For instance, transcriptional bursts have been visualized using fluorescent phage‐coat proteins that associate tightly with messenger RNA (mRNA) stem loops formed on nascent transcripts. To convert...
While flocculation has demonstrated its efficacy in enhancing yeast robustness and ethanol production, its potential application for lactic acid fermentation remains largely unexplored. Our study examined the differences between flocculating and nonflocculating Saccharomyces cerevisiae strains in terms of their metabolic dynamics when incorporating an exogenous lactic acid pathway, across varying...
Transcription presents challenges to genome stability both directly, by altering genome topology and exposing single‐stranded DNA to chemical insults and nucleases, and indirectly by introducing obstacles to the DNA replication machinery. Such obstacles include the RNA polymerase holoenzyme itself, DNA‐bound regulatory factors, G‐quadruplexes and RNA‐DNA hybrid structures known as R‐loops. Here, we...
Yeasts are naturally diverse, genetically tractable, and easy to grow such that researchers can investigate any number of genotypes, environments, or interactions thereof. However, studies of yeast transcriptomes have been limited by the processing capabilities of traditional RNA sequencing techniques. Here we optimize a powerful, high‐throughput single‐cell RNA sequencing (scRNAseq) platform, SPLiT‐seq...
The field of single‐cell omics has transformed our understanding of biological processes and is constantly advancing both experimentally and computationally. One of the most significant developments is the ability to measure the transcriptome of individual cells by single‐cell RNA‐seq (scRNA‐seq), which was pioneered in higher eukaryotes. While yeast has served as a powerful model organism in which...
Nitrogen catabolite repression (NCR) is a means for yeast to adapt its transcriptome to changing nitrogen sources in its environment. In conditions of derepression (under poor nitrogen conditions, upon rapamycin treatment, or when glutamine production is inhibited), two transcriptional activators of the GATA family are recruited to NCR‐sensitive promoters and activate transcription of NCR‐sensitive...
A constellation of mRNA particles detected by single molecule fluorescence in situ hybridization in S. cerevisiae (courtesy of Coralie Goncalves and Benoit Palancade, Institut Jacques Monod, Paris) sheds light on RNA polymerase II distribution as assessed by NET‐seq (Xi et al., Yeast, 41(4), 222–241).
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